The development, invasion and metastasis of cancer are closely interrelated with its own autocrine mechanism of growth factor, gorwth factor receptor or signal transduction. Suramin, a polyanionic compound with antiparasitic activity, has veen recently shown to have growth factor antagonist properties and to affect growth of matastic lesions of several malignancies. Chung-Ang University Penile Cancer Cell Line1( CUPE-1) is a new squamous cell carcinoma cell line which maintains morphological and functional characteristics of human pencile squamous cell carcinoma and has strong tumorigenicity when implanted into the nude mouse. Herein, an experimental study was done to investigate the effect of growth factors and suramin on the proliferation of CUPE-1 in vitro.
Of three growth factors including EGF, basic fibroblast growth factor and platelet derived gorwth factor, EGF enhanced proliferation of CUPE1 most significantly(p(0.001). Suramin of 100 g g/ml(p(0.05), 300,u g/ml and 1,000 u g/ml(p(0.001) of media inhibited in vitro proliferation of CUPE-1, significantly. In vitro antiproliferative effect of suramin on CUPE1 was reversible after stopping the drug. Suramin of 300,u g/ml inhibited the proliferation stimulating effect of EGF, signif icantly (p ( 0.001), whereas suramin of 100 u g/ml did not inhibit the effect of EGF, significantly. Suramin did not show significant cytotoxicity on H3thymidine release assay.
These results suggest that D EGF is a major growth factor of penile squamous cell carcinoma, 2) suramin is a promising drug for the treatment of advanced penile squamous cell carcinoma, 3)
food level of suramin should be continuously maintained in about 300,ug/ml in clinical trial, and 4) the main machanism cf suramin against CUPE1 is cytostatic, by antagonizing the action of .GF
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